Determination of DNA content of aquatic bacteria by flow cytometry

Article Abstract:

Flow cytometry used on water samples is as accurate as spectrophotometric analysis of cultures for measuring the DNA in bacteria. Using this technique, researchers found that the average amount of DNA in a seawater sample was 2.5 fg per cell, with 90% of the bacteria containing more than 1.1 fg per cell.

Evaluation, Measurement, DNA, Aquatic microbiology, Flow cytometry

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Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results

Article Abstract:

The development of dilution culture technique is discussed. Seawater samples were grown in dilution cultures and subjected to flow cytometry and statistical analyses. Its viability, nutritional requirements, population dynamics, allelopathic interactions and pure culture generation were emphasized in the experiments. The utility of the model for studying typical aquatic organisms was demonstrated.

Methods, Dilution, Dilution (Chemistry)

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A small, dilute-cytoplasm, high-affinity, novel bacterium isolated by extinction culture and having kinetic constants compatible with growth at ambient concentrations of dissolved nutrients in seawater

Article Abstract:

Research was conducted to examine the hypothesis that specific affinities are sufficient to support growth at ambient hydrocarbon concentrations in seawater. The specific affinity theory was extended to include constants associated with organism composition and saturation phenomena. Results demonstrate that specific affinities were protocol sensitive but under optimal conditions were 47.4 li/mg of cells/h which is a value sufficient for growth in seawater.

Microbial growth, Marine microbiology

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