A spontaneous chromosomal amplification of the ADH2 gene in Saccharomyces cerevisiae

Article Abstract:

An antimycin A-resistant mutant bearing several copies of alcohol dehydrogenase 2 (ADH2) isozymes was identified from a Saccharomyces cerevisiae strain. The added copies of ADH2 were expressed even when grown in glucose which normally inhibits expressions of ADH2 which gave an antimycin A resisting property to the mutant. Analysis of the enzyme expression indicated that a two step mechanism was involved in the ADH2 amplification. This process consisted of the insertion stage of ADH2 gene into the rDNA and the reproduction of multiple copiees of this gene due to unequal crossing over or conversion of gene within the DNA.

Mutation (Biology), Mutation

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The CCR4 protein from Saccharomyces cerevisiae contains a leucine-rich repeat region which is required for its control of ADH2 gene expression

Article Abstract:

A study was conducted to examine the CCH4 gene in Saccharomyces cerevisiae. The CCH4 gene controls transcription of the glucose-repressible alcohol dehydrogenase (ADH2). Mutations of CCH4 also influence transcription at ADH2 and his4-912delta loci as an effect of genetic defects in SPT10 (CRE1) and SPT6 (CRE2). Results show that deleting some CCH4 leucine repeat regions generates nonfunctional proteins.

Analysis, Leucine, Recombinant proteins

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Spontaneous amplification of the ADH4 gene in Saccharomyces cerevisiae

Article Abstract:

A study was conducted to analyze spontaneous gene amplification. Five ADH4 gene amplifications were identified among 1,894 antimycin A-resistant mutants. Only one of these amplifications did not exhibit 40-kb extrachromosomal palindromes which had telomere homologous sequences. Results show that ADH systems can be applied in evaluating Saccharomyces cerevisiae amplification.

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