A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters

Article Abstract:

A survey of environmental E. coli isolates from recreational waters was carried out for the first time using a DNA microarray capable of detecting all currently described virulence genes and commonly found antimicrobial resistance genes. A high proportion (29%) of 308 isolates from a beach site in the Great Lakes carried a pathotype set of virulence-related genes and 14% carried antimicrobial resistance genes, findings consistent with a potential risk for public health.

Virulence (Microbiology)

---

Waterborne pathogen detection by use of oligonucleotide-based microarrays

Article Abstract:

The detection efficacy of an oligonucleotide-based microarray designed with probes specific for the universal targets of 16S rRNA and cpn60 genes in addition to the Enterobacteriaceae-specific wecE genes of several pathogens usually encountered in wastewater is assessed. The results show the feasibility of using DNA microarrays in the detection of waterborne pathogens within mixed populations but also raise the problem of PCR bias in such experiments.

United States, Usage, Bacteria, Pathogenic, Pathogenic bacteria

---

Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli

Article Abstract:

An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes is designed and evaluated using DNA from known reference strains. This microarray is a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological and phylogenetic studies including the evaluation of genome plasticity.

Similar abstracts:

• Abstracts: Prevalence and antimicrobial resistance of thermophilic Campylobacter spp. from cattle farms in Washington State
• Abstracts: Occurrence of virulence and antimicrobial resistance genes in Escherichia coli isolates from different aquatic ecosystems within the St. Clair River and Detroit River areas
• Abstracts: 16S rRNA sequences and differences in bacteria isolated from the Muztag Ata glacier at increasing depths. Non-Frankia actinomycetes isolated from surface-sterilized roots of Casuarina equisetifolia fix nitrogen
• Abstracts: Genomic analysis of LexA binding reveals the permissive nature of the Escherichia coli genome and identifies unconventional target sites
• Abstracts: Evidence of intense archeal and bacterial methanotrophic activity in the Black Sea water column. Cooccurrence of aerobic and anaerobic methane oxidation in the water column of Lake PluBsee

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.