Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study

Article Abstract:

Results show that approximately 150 to 180 extracellular proteins are produced by Bacillus subtilis as revealed by two-dimensional electrophoretic and computer search studies.

Japan, Statistical Data Included, Usage, Physiological aspects, Gel electrophoresis, Proteins, Secretion, Physiological regulation

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Acquisition of azide-resistance by elevated SecA ATPase activity confers azide-resistance upon cell growth and protein translocation in Bacillus subtilis

Article Abstract:

Amino acid substitution of threonine 128 of SecA by alanine or isoleucine Bacillus subtilis yields four azide-resistant Sec A mutants that exhibit normal protein translocation and cell growth in the presence of 1.5 millimoles of sodium azide, unlike the wild-type strain that is inhibited. Purified mutant SecA proteins have three times higher values of basal and elevated level ATPase activities than the wild-type SecA. The decrease in ATPase activity of the mutants when 1.5 millimoles sodium azide is added is less than that in the wild-type, indicating that protein translocation in B. subtilis requires elevated ATPase activity, which is resistant to sodium azide in the mutants.

Research, Adenosine triphosphatase, Microbial mutation

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Determination of a 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome

Article Abstract:

A 21,548 bp nucleotide sequence, which is 306 kb downstream from the zero point of the physical map and in the vicinity of the 24 degree region of the Bacillus subtilis chromosome, contains 21 putative open reading frames (ORFs). Two of them match the amino acid and nucleotide sequence of B. subtilis orfJ and Pcp while four others are similar to known proteins in data banks. Three ORFs resemble the sensor-regulator proteins of bacterial two-component signal transduction systems. Six ORFs have been sequences that do not resemble any known protein.

Genetic aspects, Nucleotide sequence, Base sequence

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