Advances in development of a genetic system for Thermoanaerobacterium spp.: expression of genes encoding hydrolytic enzymes, development of a second shuttle vector, and integration of genes into the chromosome

Article Abstract:

Advances in the genetic engineering of Thermoanaerobacterium spp. are discussed. This organism can survive at temperatures up to 80 deg. C. and can use xylan, which is a cheap substrate for industrial fermentation.

Microbial genetic engineering, Bacteria, Thermophilic, Thermophilic bacteria

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A high-molecular-weight, cell-associated xylanase isolated from exponentially growing Thermoanaerobacterium sp. strain JW/SL-YS485

Article Abstract:

Gel filtration suggests a molecular weight of 350 kDa for cell-associated beta-1,4-xylanase isolated from the cell extract of the exponentially growing Thermoanaerobacterium sp. strain JW/SL-YS485, while gel electrophoresis suggests a molecular weight of 234 kDa for beta-1,4-xylanase. Enzyme assay reveals that beta-1,4-xylanase exhibits highest activity at pH 6.2 and 80 degrees Celsius. Size, location and subunit structure of beta-1,4-xylanase are different from those of other xylanases.

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Purification and characterization of the alpha-glucuronidase from Thermoanaerobacterium sp. strain JW/SL-YS485, an important enzyme for the utilization of substituted xylans

Article Abstract:

Chromatographic techniques help purify cell-associated alpha-glucuronidase from the Thermoanaerobacterium sp. strain JW/SL-YS485. Analysis of the enzyme reveals the molecular weight as 130,000 and it has a pI of 4.65. Enzyme assay reveals that the enzyme exhibits optimum activity at pH 5.4 and 60 degrees Celsius. 4-methylglucuronosyl xylotetraose is synergistically hydrolyzed by alpha-glucuronidase and beta-xylosidase of the same organism.

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