Anaerobic oxidations of cysteate: degradation via L-cysteate: 2-oxoglutarate aminotransferase in Paracoccus pantotrophus

Article Abstract:

A study was conducted to analyze the range of anaerobic oxidations of cysteate with environmental electron acceptors, nitrate, sulfate and iron in Paracoccus pantotrophus. The enrichment cultures were prepared in a basal salts medium. Growth experiments with strain NKNCYSA were carried out in a medium supporting the reductant titanium(III) nitrilotriacetate. Results indicated that the cell free extracts of P pantotrophus supported constitutive L-cysteate:2-oxoglutarate aminotransferase and glutamate dehydrogenase.

Anaerobic bacteria, Aminotransferases, Transaminases

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Thioredoxin is essential for Rhodobacter sphaeroides growth by aerobic and anaerobic respiration

Article Abstract:

A thioredoxin-deficient mutant of Rhodobacter sphaeroides was used to study the functional activity of thioredoxin. In situ and Southern hybridization assays revealed that deficiency in thioredoxin gene is lethal for the growth of the bacteria in anaerobic conditions with DMSO as terminal electron acceptor and in aerobic conditions. It was also found that R. sphaeroides thioredoxin exhibits some GSH-disulfide oxidoreductase activity thus, explaining its significance in maintaining the growth of the bacteria.

Nucleic acid hybridization, In situ hybridization, Proteins, Microbial respiration

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Isolation of an extracellular protease gene of Erwinia carotovora subsp. carotovora strain SCC3193 by transposon mutagenesis and the role of protease in phytopathogenicity

Article Abstract:

The isolation of a mutant gene from Erwinia carotovora subsp. carotovora SCC3193 via transposon mutagenesis was conducted. The resulting mutation, designated as SCC6004, exhibited normal production levels of polygalacturonase, cellulase and pectate lyase. A complete ORF, designated as prtW, was observed to be strongly induced in the presence of plant extracts. The mutant's reduced virulence indicates that prtW increases the ability of SCC3193 to destroy plant tissue.

Plant-pathogen relationships, Plant diseases, Transposons

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