Analysis of beta-subgroup proteobacterial ammonia oxidizer populations in soil by denaturing gradient gel electrophoresis analysis and hierarchical phylogenetic probing

Article Abstract:

Research was conducted to examine the influence of soil pH on the compositions of natural populations of autotrophic beta-subroup proteobacterial ammonia oxidizers. A combination of oligonucleotide probing and denaturing gradient gel electrophoresis (DGGE) was used with objective of investigating the hypotheses that Nitrosospira may be significant for ammonia oxidation in acid soils and that the relative abundances of various sequence clusters are related to soil pH. Results reveal the value of DGGE and suggests that Nitrosospira cluster 2 may be important for ammonia-oxidizing activity in acid soils.

Gel electrophoresis, Nucleic acid probes, Ammonia, Hydrogen-ion concentration, Soil acidity

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Nitrogen cycling and community structure of proteobacterial beta-subgroup ammonia-oxidizing bacteria within polluted marine fish farm sediments

Article Abstract:

16S rDNA techniques were used in studying the effects of organic particulate matter from a marine fish farm on the ammonia-oxidizing bacteria community in the underlying sediment. Organic content, ammonium concentrations, nitrification rates and ammonia oxidizer most-probable-number counts were determined in sediment samples. Results reveal inhibition of nitrification and denitrification directly beneath the fish cage. A Nitrosomonas group induced by the extent of the pollution was observed in the bacteria community based on band migration, hybridization and sequencing.

Environmental aspects, Bacteria, Microbial mats, Marine pollution, Nitrogen cycle

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Molecular diversity of soil and marine 16S rRNA gene sequences related to beta-subgroup ammonia-oxidizing bacteria

Article Abstract:

The 16S rRNA gene sequences of the ammonia-oxidizing beta-proteobacteria in soil and marine samples can be separated into Nitrosospira genus-like sequences and Nitrosomonas genus-like sequences. The bacteria in environmental samples form seven clusters and those in soil form four clusters. Each of the clusters consist of varied proportions of acid and neutral soil bacteria. The sequences of the soil sediment libraries differ from the marine sediment libraries. The degree of pollution fails to determine the community composition.

Observations, Soil microbiology, Ribosomal RNA, Bacteria, Nitrifying, Nitrobacteraceae

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