Analysis of mechanisms regulating expression of the ver-1 gene involved in aflatoxin biosynthesis

Article Abstract:

A systematic analysis of aflatoxin biosynthesis was conducted in Aspergillus parasiticus to determine the molecular mechanisms that regulates the accumulation of proteins encoded by aflatoxin biosynthetic genes. Analysis of aflatoxin biosynthesis in Aspegillus parasiticus indicated the similar appearance of GUS transcript and GUS proteins with the accumulation of ver-1A transcript and Ver-1 protein. Furthermore, gene expression levels in Aspergillus parasiticus are dependent on chromosomal location and the mechanisms for gene inactivation exists in the fungi strain.

Genetic aspects, Microbiological synthesis, Fungi

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Enzymatic function of the Nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus

Article Abstract:

The enzymatic function of the Nor-1 protein in aflatoxin biosynthesis has been studied in Aspergillus parasiticus. The nor-1 gene is involved in aflatoxin biosynthesis in A. parasiticus and has been predicted to encode a norsolorinic acid ketoreductase. Recombinant Nor-1 expressed in Escherichia coli changed the 1' keto group of norsolorinic acid to the 1' hydroxyl group of averantin in crude E. coli cell extracts where NADPH is present. Results show that in vitro nor-1 acts as a ketoreductase.

United States, Statistical Data Included, Research, Physiological aspects, Enzymes, Biosynthesis, Bacterial proteins, Molecular microbiology

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