Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum

Article Abstract:

The viability of antisense RNA in redirecting the primary metabolism of Clostridium acetobutylicum has been studied. C. acetobutylicum is a gram-positive, spore-forming anaerobe that can ferment various sugars into acids and solvents. Two plasmid constructs that can produce the asRNA complementary to either the ptb gene or the buk gene have been constructed. The strain ATCC 824(pRD4) contains a 102-nucleotide asRNA with 87% complementarity to the butyrate kinase gene. The ATCC 824(pRD1) strain has a 698-nucleotide as RNA with 96% complementarity to the PTB gene.

Physiological aspects, Clostridium, Antisense RNA

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Genetic characterization and physiological role of endopeptidase O from Lactobacillus helveticus CNRZ32

Article Abstract:

Research was conducted to examine the characterization of a metalloendopeptidase gene (pepO) of CNRZ323 and the characterization and construction of derivatives lacking PepO. A 1,94-bp pepO ORF with an upstream AT-rich sequence, which might act as the putative -10 and -35 promoter regions, was identified by nucleic acid sequencing. Results indicate that the pepO and pepO pepX strains did not exhibit significant differences in their acidification or growth rates from those of the wild type and pepX mutant, respectively.

Proteases, Lactobacillus, Insertion elements, DNA, DNA insertion elements

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Analysis of mer gene subclasses within bacterial communities in soils and sediments resolved by fluorescent-PCR-restriction fragment length polymorphism profiling

Article Abstract:

A research was conducted to examine the application of the mer gene model system in studying the genetic diversity in bacterial community sites in order to define the spatial profiles of mer genes in natural environments. Fluorescent-polymerase chain reaction-restriction fragment length polymorphism was utilized to profile the mer gene. Results suggested different spatial dimensions to the distribution of a specific bacterial gene in soil and sediment environments.

Usage, Operons, Polymorphism (Crystallography)

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