Genetically controlled self-aggregation of cell-surface-engineered yeast responding to glucose concentration

Article Abstract:

Researchers have engineered a strain of yeast that displays a-agglutinin and alpha-agglutinin on the cell surface. This yeast grows normally at high glucose concentrations, but agglutinates and sediments completely when glucose concentrations are very low. This can be used industrially to separate the cells from the supernatant.

Microbial genetic engineering, Yeast, Yeast (Food product), Industrial microbiology

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Construction of a starch-utilizing yeast by cell surface engineering

Article Abstract:

The cell surface of the yeast Saccharomyces cerevisiae was engineered by anchoring active glucoamylase protein on the cell wall. The yeast cells were given the capacity to use starch directly as the only carbon source. By employing yeast alpha-agglutinin as an anchor region and the secretion signal peptide from Rizhopus oryzae glucoamylase, glucoamylase was transported and covalently bound to the cell surface.

Starch

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Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing beta-glucosidase and carboxymethylcellulase from Aspergillus aculeatus

Article Abstract:

A study was conducted to characterize the genetic immobilization of beta-glucosidase on the Saccharomyces cerevisiae cell surface in addition to the CMCase. The assimilation of cellobiose and cellooligosaccharides by the recombinant yeast was also examined. Escherichia coli was utilized as a host for recombinant DNA manipulation. Results indicated that enzymes on the cell surface of S. cerevisiae may support the uptake of glucose liberated on the cell surface.

Escherichia coli, Aspergillus, Genetic recombination, Glucosidases, Alpha glucosidases

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