Genomic structure of phage B40-8 of Bacteroides fragilis

Article Abstract:

Research was conducted to examine the molecular characterization of phage B40-8 of Bacteroides fragilis HSP40. Three major proteins out of 15 peptides in the SDS-PAGE profile were chosen for N-terminal sequencing. Immunodetection by electron microscopy provide evidence that major protein (MP)1 and MP3 were structural proteins of the phage head and that MP2 was a constituent of the tail. The structure of the genome and the localization of the major proteins in the genomic restriction map of the phage are described.

Genomes, Bacterial proteins

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Site-specific integration of corynephage phi16: construction of an integration vector

Article Abstract:

A temperate phage, phi16, produced from Corynebacterium glutamicum ATCC 21792, was found to lysogenize its host through site-specific recombination. The phage attachment location, attP, was localized to a 6.5 kb BamHI fragment of the phi16 genome. This fragment also held phi16 integrative functions. The comparison of the nucleotide sequences of attB, attL, attR and attP indicated a common 29 bp sequence, the core sequence. It is found 11 bp downstream of the 3' end of the integrase gene.

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