Binding of the CryIVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins

Article Abstract:

Environmentally safe biopesticides are the subject of much study and of these, bacillus thuringiensis represents a promising one. The CryIVD toxin of Bacillus thuringiensis subsp. israelensis binds to the proteins of 148 kDa in Anopheles stephensi and of 78 kDa in Tipula oleracea, as indicated by the ligand-blotting experiments on dipteran brush border membrane vesicles (BBMV). Liability towards the CryIVD toxin is 10000 times more for larvae of A. stephensi than for T. oleracea. In the BBMV of lepidopteran species Manduca sexta, CryIVD binds to six proteins ranging from 48 to 110 kDa, but CryIVD is not toxic here.

Analysis, Observations, Carrier proteins, Transport proteins, Toxins

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High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20 kilodalton protein, and the coexprssed gene products are synergistic in their toxicity to mosquitoes

Article Abstract:

The insecticidal activities of genes constructed from Eschirichia coli or Bacillus thuringiensis was studied. These interactions were determined by the derivation of different DNA fragments from the 9.4 kb HindIII of pMI which was introduced into a shuttle vector of B. thuringiensis for expression. Electrophoretic and immunoblot analyses were to characterize the resulting proteins. A synergistic interaction was observed.

Genetic aspects, Proteins, Insect pests, Biological insect control

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