Diversity of proteolytic Clostridium botulinum strains, determined by a pulsed-field gel electrophoresis approach

Article Abstract:

An optimized pulsed-field gel electrophoresis (PFGE) protocol is used to study the strains of proteolytic Clostridium botulinum from various origins in order to learn about the biodiversity of proteolytic C. botulinum and evaluate the usefulness of PFGE in the genotyping of this organism. The results show that PFGE is considered to be a useful tool for molecular epidemiological analysis of proteolytic C. botulinum types A and B.

Science & research, Research, Gel electrophoresis

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Efficient DNA fingerprinting of Clostridium botulinum types A, B, E, and F by amplified length polymorphism analysis

Article Abstract:

The applicability of amplified fragment length polymorphism (AFLP) analysis in characterization of Clostridium botulinum groups I and II are assessed and the genetic biodiversity of C. botulinum types A, B, E, and F are analyzed. The results show that AFLP is a fast, highly discriminating and reproducible DNA fingerprinting method with excellent typeability that can be also be used for C. botulinum group identification.

Analysis, Genetic aspects, DNA testing, DNA identification, Genetic polymorphisms

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