Biosynthesis of novel exopolymers by Aureobasidium pullulans

Article Abstract:

Biosynthesis of novel exopolymers by Aureobasidium pullulans has been investigated using A. pullulans ATCC 42023 cultured under aerobic conditions to find biosynthesis methods to modulate composition and structural features of exopolymers and gain knowledge of metabolic flexibility of the biosynthesis/secretion pathways. Mannose, glucose and glucose analogs were energy sources. Exopolymer extracts were made up of mannose and glucose with the molar ratio in the exopolymer extract and the molecular weight of the exopolymer dependent on energy source and culture time. Glucose content of exopolymer extracts formed with mannose and glucose as carbon sources was 91-87%.

United States, Statistical Data Included, Drugs, Physiological aspects, Bacteria, Fermentation, Cytochemistry, Polysaccharides, Food preservatives

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Production of R-(-)-ketoprofen from an amide compound by Comamonas acidovorans KPO-2771-4

Article Abstract:

The bacterial strain Comamonas acidovorans KPO-2771-4 produces R-(-)-2-(3'-benzoylphenyl)propionic acid (R-(-)-ketoprofen) from racemic 2-(3'-benzoylphenyl)propionamide (keto-amide). The organism requires sodium fumarate as a carbon source and 2-azacyclononanone or isobutyronitrile as catalysts for growth and formation of R-(-)-ketoprofen. The R-Amidase activity, which is rare in R-keto-amide-utilizing microorganisms, is present in C. acidovorans as the keto-amide is hydrolyzed to R-ketoprofen and ammonia in its presence.

Usage, Amides, Ketoprofen

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Cometabolism of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene by Pseudomonas acidovorans M3GY grown on biphenyl

Article Abstract:

A study was conducted to investigate the ability of Pseudomonas acidovorans M3GY grown on biphenyl to alter 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) and its unchlorinated analog, 1,1-diphenylethylene. Culture samples were centrifuged while metabolites were determined from cells cultured in the dark for a minimum of 25 days. Results showed that DDE is attacked by a dioxygenase at the ortho and meta positions based on the recovery of a metabolite from neutral extracts.

Pseudomonas, DDT (Insecticide), DDT, Biphenyl compounds, Biphenyls (Class of compounds), Cometabolism

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