Biphasic extracellular proteolytic enzyme activity in benthic water and sediment in the northwestern Mediterranean Sea

Article Abstract:

The maximal biphasic extracellular ectoproteolytic enzyme activities and affinities of natural benthic microbial communities have been investigated using the multiconcentration kinetic approach. A set of 36 samples of near-bottom water and sediment collected from a coastal area and an offshore area in the northwestern part of the Gulf of Lions was analyzed. The existence of biphasic microbial ectoproteolysis was statistically confirmed for both the near-bottom sediment and water, regardless of the seasonal and spatial conditions. It was also shown that deep benthic bacterial consortia exhibited better response with episodic nutrient supplies than shallower benthic bacterial consortia.

Analysis, Water, Aquatic microbiology, Proteolysis, Benthos, Benthic zone, Sediments (Geology)

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Organic solvent-tolerant bacterium which secretes an organic solvent-stable proteolytic enzyme

Article Abstract:

The Pseudomonas aeruginosa strain PST-01 is able to grow in a medium containing organic solvents and produces a proteolytic enzyme. The strain is obtained by isolating proteolytic enzyme producers and then selecting organic solvent-tolerant microorganisms from these enzyme producers. Cyclohexane suppresses the formation of the enzyme. The optimum temperature for the enzyme is 55 degrees celsius and optimum pH is 8.5. The proteolytic activity is stable in the presence of various organic solvents and is similar to the activity in the absence of organic solvents.

Observations, Organic solvents

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Role of intermolecular disulfide bonds of the organic solvent-stable PST-01 protease in its organic solvent stability

Article Abstract:

The disulfide bond between Cys-270 and Cys-279 of the PST-01 protease of Pseudomonas aeruginosa is essential for activity and the disulfide bond between Cys-30 and Cys-58 contribute to its stability in organic solvents. The protease could be useful for reactions that take place in organic solvents.

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