Carbapenem antibiotic production in Erwinia carotovora is regulated by CarR, a homologue of the LuxR transcriptional activator

Article Abstract:

Molecular genetic analysis of group 1 mutants of the carbapenem antibiotic-producing Erwinia carotovora GS101 involves the isolation of a complementary cosmid cWU142. Sequence analysis of a cWU142 subclone reveals that the carR gene codes for a homologue of the Vibrio fischeri LuxR protein. The carR gene serves as a hot spot target for a Tn5 element transposition, leading to the formation of carR mutants at a high frequency. Genes that code for several metabolic functions such a plasmid conjugation, bioluminescence and cell division are transcriptionally induced by the carR protein, which depends on N-acyl homo-serine lactones for its function.

Physiological aspects, Gram-negative bacteria, Bacterial proteins

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Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage phiM1

Article Abstract:

The isolation and characterization of the first generalized transducing phage for Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043 was reported. It was found that the phage (phiM1) infects a large spectrum of Eca strains which could be useful for the general genetic analysis of many Eca isolates and for the transductional transfer of selectable markers between strains.

Bacteriophages, Transduction, Transduction (Genetics)

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A pheromone-independent CarR protein controls carbapenem antibiotic synthesis in the opportunistic human pathogen Serratia marcescens

Article Abstract:

A carbapen-2-em-3-carboxylic acid (CarR) protein with homology to the LuxR family of transcriptional regulators is encoded to isolate and identify a Serratia marcescens carR gene. Test results show that this protein is capable of activating carbapenem synthesis in the said bacteria. Moreover, CarR can release a heterologous Erwinia carotovora host for carbapenem production.

Bacterial genetics, Pathogenic microorganisms

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