Characterization of Shiga toxins gene (stx)-positive and intimin gene (eae)-positive Escherichia coli isolates from wastewater of slaughterhouses in France

Article Abstract:

Wastewater samples from 12 slaughterhouses located in different regions in France are tested for the presence and characteristics of stx-positive and eae-positive Escherichia coli isolates. The results have shown that the majority of the stx- and eae-positive E. coli isolates from wastewater have low virulence for humans, but they have helped in determining the role of the environment in the emergence of new pathogenic Shiga toxin [Stx]-producing E. coli (STEC) and enterohemorrhagic E. coli (EHEC) strains.

France, Pollutants produced & recycled, Sewage, Waste management, Sewage treatment, Slaughtering and slaughter-houses, Slaughterhouses

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Comparative proteomic analysis of extracellular proteins of enterohemorrhagic and enteropathogenic Escherichia coli strains and their ihf and ler mutants

Article Abstract:

A comparative proteomic approach is used to analyze the extracellular proteomes of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli in order to give insight into the pathogenesis and divergence of these two emerging pathogens. The results show that proteomics is a powerful platform technology for accelerating the understanding of EPEC and EHEC pathogenesis and identifying markers for laboratory diagnoses of these pathogens.

Microbiological research, Proteomics

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Transduction of porcine enteropathogenic Escherichia coli with a derivative of a shiga toxin 2-encoding bacteriophage in a porcine ligated Ileal loop system

Article Abstract:

An in vivo transduction model for wild type E.coli strains examines the putative role of EPEC for the evolution of EHEC by acquisition of stx2 genes by utilizing a pig ligated ileal loop system. The results indicate that in vivo conditions are more effective for transduction of Stx2 encoding phages than in vitro conditions.

Transduction, Transduction (Genetics)

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