Characterization of form variants of Xenorhabdus luminescens

Article Abstract:

Xenorhabdus spp. are entomopathogenic bacteria which forms symbiotic complexes with nematodes of the genera Steinernama and Heterorhabditis. Becauseof these complexes' potential as biological control agents, the form variants of X. luminescens XE-87.3 were characterized. Four form variants were isolated and found to differ in their pigmentation and antibiotic production. The four form variants could not be distinguished by DNA fingerprinting, indicating a clonal deriviation. However, a few differences were detected in their protein patterns, while their pathogenicity differed significantly.

Virulence (Microbiology), Variation (Biology)

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Comparative analysis of antibiotic resistance, immunofluorescent colony staining, and a transgenic marker (bioluminescence) for monitoring the environmental fate of a rhizobacterium

Article Abstract:

Field releases of the wild-type plant growth-promoting rhizobacterium Pseudomonas fluorescens 89B-27, its bioluminescent derivative GEM-8 and a spontaneous rifampicin-resistant variant (R34) were established on cucumber. These were used to examine the efficacy of these marker systems for estimating the wild-type populations. Findings revealed that conventional marker systems may underestimate populations and hence the survival and colonization of genetically marked bacteria.

Antibiotics, Drug resistance in microorganisms, Microbial drug resistance

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Polyclonal antisera to distinguish strains and form variants of Photorhabdus (Xenorhabdus) luminescens

Article Abstract:

Serological techniques use a new polyclonal antisera to differentiate between different strains and form variants of Photorhabdus luminescens. In the double-diffusion test antiserum 9226 exhibits different reactivity with different strains, revealing the serological difference between the strains. Cross-absorption increases the specificity of antisera, and primary and secondary forms of P. luminescens are discriminated using cross-absorbed antisera.

Morphology (Biology), Immune serums

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