Characterization of the genetic locus responsible for production and immunity of carnobacteriocin A: the immunity gene confers cross-protection to enterocin B

Article Abstract:

The immunity protein CbiA of Carnobacterium piscicola LV17A also protects against enterocin B as well as carnobacteriocin A and vice versa. Immunity proteins are proteins that protect bacteria from their own bacteriocins.

Bacterial toxins

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Expression of the antimicrobial peptide carnobacteriocin B2 by a signal peptide-dependent general secretory pathway

Article Abstract:

Strains of Carnobacterium divergens, LV13 and C. piscicola produce carnobacteriocin B2 through a general signal peptide-dependent secretory pathway. Carnobacteriocin B2 is a broad spectrum bacteriocin that is active against lactic acid bacteria. The export of this bacteriocin depends on specific ATP-binding cassette secretion proteins. C.divergens LV13, which produces divergicin A and carnobacteriocin B2, exhibits multiple bacteriocin expression through the general secretory pathway.

Analysis, Anti-infective agents, Bacteria, Peptides

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Atypical genetic locus associated with constitutive production of enterocin B by Enterococcus faecium BFE 900

Article Abstract:

Enterocin B produced by Enterococcus faecium T136 from meat has been shown to be similar to a purified bacteriocin produced by Enterococcus faecium BFE 900 synthesized from black olives. This is evident in Edman degradation and mass spectrometric analyses of BFE 900, which identified the location of the structural gene to be on a 2.2-kb HindIII fragment and a 12.0-kb EcoRI chromosomal fragment. The enterocin B genetic system differs from most other bacteriocin systems with respect to presence of transport and other genes linked to bacteriocin production on the 12.0-kb chromosomal fragment having the enterocin B structural gene.

Plasmids, Bacterial genetics, Genetic code, Microbial genetics

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