Characterization of two autoreplicative regions of the IncHI2 plasmid R478:RepHI2A and RepHI1A(sub.R478)

Article Abstract:

Two autoreplicative regions of the incompatibility group HI2 (IncHI2) plasmid R478 have been characterized, the first autoreplicative regions from an IncHI2 subgroup plasmid to be described. The regions are RepHI2A and RepHI1A(sub.R478). The prototype IncHI2 subgroup plasmid, R478, has been shown to have at least two iteron-controlled autoreplicative regions. The presence of RepHI1A(sub.R478) on R478 likely explains the incompatibility seen between R478 and plasmids of the IncHI1 subgroup. Plasmids in the IncH encode resistance determinants and have been associated with many Salmonella-related epidemics and pandemics.

Ireland, Disease transmission, Plasmids, Salmonella, Molecular biology

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Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system

Article Abstract:

Microbial consortia in a terephthalate-degrading anaerobic granular sludge system have been characterized using a molecular approach along with electron microscopy in a laboratory-scale terephthalate-degrading UASB reactor. It ws found that 78.5% of 106 bacerial clones were from the delta subclass of Proteobacteria and most of these were in a novel group with no known bacterial isolates. The other clones were from the green nonsulfur bacteria, Synergistes and unidentified groups.

Taiwan, Usage, Plastics industry, DNA, Bioremediation, Gel electrophoresis, Cloning, Anaerobic bacteria, Terephthalic acid, Polyester industry

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Microbial diversity in marine sediments from Sagami Bay and Tokyo Bay, Japan, as determined by 16s rRNA gene analysis

Article Abstract:

Research conducted with197 16S rDNA clones showed that the clone composition differed at each of the sampling sites (SA, SB and TK) with a genotypic diversity of 0.958, 0.636 and 0.821 respectively. The rDNA sequence data identify bacteria having derived from five major lineages, and gamma Proteobacteia and high-GC gram-positive bacteria were dominant.

Japan, Analysis, Genotype, Genotypes, Microbial ecology, Ribosomal RNA, Microbial populations, Tokyo Bay

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