Cloning and sequencing of pepC, a cysteine aminopeptidase gene from Lactococcus lactis subsp. cremoris AM2

Article Abstract:

The cloning and sequencing of the gene encoding the aminopeptidase PepC from L. lactis subsp. cremoris AM2 is discussed. The genetic determinant encoding aminopeptidase PepC was cloned by complementation of the pepN gene from Eschirichia coli 1047. The clones were subjected to DNA sequence analysis and were searched for protein sequences showing homology to the deduced protein encoded by open reading frame. Results indicate an intracellular location of the enzyme as no signal sequence was found.

Methods, Genetic aspects, Cloning, Aminopeptidases, Cysteine

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Oligopeptides are the main source of nitrogen for lactococcus lactis during growth in milk

Article Abstract:

Oligopeptides appear to be the primary nitrogen source for Lactococcus lactis growing in milk. The growth in milk of L. lactis strains with and without proteinase revealed that both appeared to use oligopeptides as opposed to free amino acids or di- and tripeptides. Strains deficient in oligopeptide transport had impaired growth in milk. The strains with proteinase exhibited a second growth phase that involved the hydrolysis of milk proteins to yield oligopeptides.

Analysis, Usage, Peptides, Amino acids, Milk, Oligopeptides

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