Cloning of a creatinase gene from Pseudomonas putida in Escherichia coli by using an indicator plate

Article Abstract:

An alternative strategy to screen large numbers of transformants for creatinase activity was developed. The procedure involves the coculture of transformants with Proteus mirabilis to utilize its urea-hydrolyzing ability as an inducer of color change in indicator plates. The procedure was used to screen an Pseudomonas putida NTU-8 genomic library, resulting in the isolation of a 2.7-kb DNA fragment coding for creatinase activity.

Methods, Enzymes, Cloning

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Engineering of solvent-tolerant Pseudomonas putida S12 for bioproduction of phenol from glucose

Article Abstract:

An entirely biobased production process for the conversion of a renewable substrate (glucose) into the toxic bulk chemical phenol via the central metabolite tyrosine is achieved in the solvent-tolerant strain Pseudomonas putida S12. The method results in accumulation of 58 mM phenol in the octanol phase and there is a twofold increase in the overall production compared to a single-phase fed batch.

Phenols, Phenols (Class of compounds), Glucose, Chemical properties, Dextrose

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Chromosomal locus for cadmium resistance in Pseudomonas putida consisting of a cadmium-transporting ATPase and a MerR family response regulator

Article Abstract:

Pseudomonas putida contains two proteins that confer resistance to cadmium. CadA is similar to cadmium-transporting ATPases in gram-positive bacteria and CadR is similar to the MerR family involved in mercury detoxification. CadA is induced by cadmium, lead, and zinc whereas CadR is only induced by cadmium.

Research, Heavy metals

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