Comparative physiological studies on hyperthermophilic Archaea isolated fromdeep-sea hot vents with emphasis on Pyrococcus strain GB-D

Article Abstract:

Three new bacterial isolates collected from the Guaymas Basin vent site were physiologically characterized and compared to previously characterized deep-sea vent hyperthermophiles. The results showed that temperatures greater than 105 degrees celsius were growth limiting, while none of the isolates survived temperatures of 150 degrees celsius or higher. Simulation of in situ pressure resulted in increases in growth rates at optimum growth temperatures. Oxygen toxicity depended on the isolate and the growth temperature, with the non-sulfur-dependent isolate beingthe most oxygen tolerant.

Bacteria, Thermophilic, Abyssal zone, Microorganisms, Temperature effects, Thermophiles

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A pressurized chemostat for the study of marine barophilic and oligotrophic bacteria

Article Abstract:

A low-cost pressurized chemostat is proposed for growing and analyzing marine barophilic and oligotrophic bacteria in steady-state populations. The chemostat is provided with a backpressure regulating pump which offers an accuracy of more than 1.0%. It also lacks a gas phase, eliminating cumbersome safety precautions. The device can be modified for use in the growth studies of hyperthermophilic bacteria and archae.

Equipment and supplies, Design and construction, Bacterial genetics, Chemostat

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Effects of dissolved sulfide, pH, and temperature on growth and survival of marine hyperthermophilic archaea

Article Abstract:

The ability of metabolically diverse hyperthermophilic archaea to withstand the high temperatures, low pHs, high sulfide concentrations, and the absence of carbon and energy sources was investigated. The organisms tolerated greater extremes of low pH, high sulfide concentration, and high temperature when actively growing and metabolizing than when starved of carbon sources and electron donors/acceptors.

Science & research, Physiological aspects, Archaeabacteria, Electron donor-acceptor complexes, Hydrogen-ion concentration, pH, Archaea

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