Comparative study of 35 bacteriophages of Lactobacillus helveticus: morphology and host range

Article Abstract:

Thirty five bacteriophages of Lactobacillus helveticus were studied in regard to their morphological characteristics and relationships between morphotypes, origin and host ranges. All of the phages had a contractile sheath with either long or short tails. Based on host range, three phage groups were delimited, while the bacterial strains were classified into two bacterial groups based on strain sensitivity patterns. The results also indicated that lysogenic strains could be the major source of phages in French cheese factories.

Observations, Bacteriophages, Lysogeny

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Localization of peptidases in Lactococci

Article Abstract:

The localization of five peptideaes in Lactococcus lactis was studied. These peptides, which include an endopeptidase, tripeptidase, an X-prolyl-dipetidyl aminopeptidase (XPDAP) and two aminopeptidase, were analysed by immunonoblotting after cell fractionation and by electron microscopy of immunogold-labeled peptidases. Results showed that all the peptidases are intracellular. However, peripheral location was also detected for the endopeptidase, the XPDAP, and the tripeptidase.

Analysis, Proteases

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A Lactobacillus helveticus-specific DNA probe detects restriction fragment length polymorphisms in this species

Article Abstract:

A 2-kb fragment derived from plasmid CNRZ 1094 was tested for its potential as an oligonucleotide probe to identify and classify Lactobacillus helveticus based on restriction fragment length polymorphisms. Southern hybridization tests on various species confirmed the probe's specificity to L. helveticus. Furthermore, probing of EcoRI digests of L. helveticus DNA showed restriction fragment length polymorphisms which could be used for subsequent cluster analysis.

Evaluation, Lactobacillus, Identification and classification, DNA probes

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