Comparison of an in vitro method and an in vivo method of Giardia excystation

Article Abstract:

In vitro and in vivo methods of excystation were compared to determine the more useful method of retrieving Giardia duodenalis isolates. In both whole set and within-set replicated experiments, the in vivo method produced 100% excystation while the in vitro method produced 76-83%. Bacterial contamination accounted for 23% and 17% loss in the in vivo and in vitro methods, respectively. Assessment of culture retrieval showed that adaptation was greater in the in vivo (29%) than the in vitro (15%) method. Although the differences between methods were not statistically significant, overall retrieval rates suggest the in vivo method to be superior.

Growth, Giardia, Giardia lamblia, Giardiasis

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Grazing pressure by a bacterivorous flagellate reverses the relative abundance of Comamonas acidovorans PX54 and Vibrio strain CB5 in chemostat cocultures

Article Abstract:

Grazing pressure from a bacterivorous flagellate produced remarkable changes in the cell morphology and the abundance of the bacterial strains Comamonas acidovorans PX54 and Vibrio strain CB5 in chemostat experiments. In previous studies, similar changes were observed upon the introduction of protozoan grazing. Changes in the two strains' relative abundances are believed to have been caused by a combination of increasing growth rates resulting from flagellate grazing, the ability of the species to add cell length, and size-selection in flagellate grazing.

Bacteria, Flagella (Microbiology), Flagella

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Enrichment of mixed cultures capable of aerobic degradation of 1,2- dibromoethane

Article Abstract:

A mixed culture aerobically degrades 1,2-Dibromoethane (DBE), leading to the formation of biodegradation products and the disappearance of DBE from the biological medium. DBE is a carcinogenic environmental contaminant, present in soil and groundwater supplies. Bromoethanol is an intermediate in the DBE degradation. The bromoethanol degradation involves the degradation of bromoacetate to bromoacetaldehyde.

Analysis, Biodegradation, Ethylene dibromide

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