Competitive metagenomic DNA hybridization identifies host-specific microbial genetic markers in cow fecal samples

Article Abstract:

The application of a genome fragment enrichment (GFE) method is described to identify host-specific genetic markers from fecal microbial community DNA. The data demonstrate that direct metagenomic DNA genomic analysis by the competitive solution hybridization approach is an efficient method for identifying potentially useful fecal genetic markers and for characterizing differences between environmental microbial communities.

Research and Development in the Physical, Engineering, and Life Sciences, Administration of Air and Water Resource and Solid Waste Management Programs, Water Pollution Control R&D, Water Pollution R&D, Methods, Nucleic acid hybridization, Water pollution research

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Old yellow enzymes protect against acrolein toxicity in the yeast Saccharomyces cerevisiae

Article Abstract:

The complete set of viable deletion strains in Saccharomyces cerevisiae is screened for sensitivity to acrolein for identifying cell functions involved in resistance to reactive aldehydes. It is shown that old yellow enzyme 2 (Oye 2) which is a widely conserved NADPH-dependent oxidoreductase protects against acrolein toxicity in the yeast Saccharomyces cerevisiae.

Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Brewers' Yeast, Physiological aspects, Genetic aspects, Eukaryotes, Brewer's yeast, Saccharomyces cerevisiae, Coenzymes, Oxidative stress

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Application of genome-wide expression analysis to identify molecular markers useful in monitoring industrial fermentations

Article Abstract:

Molecular markers useful in monitoring industrial fermentations specifically for conditions of zinc deficiency are identified. The results indicate that these sequences may be valuable molecule markers for detecting zinc deficiency in industrial fermentations.

Microbiological research

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