Conidial hydrophobins of Aspergillus fumigatus

Article Abstract:

The outer cell wall layer of Aspergillus fumigatus exhibit two hydrophobins, RodAp and RodBp, and they are insoluble complexes. Research reveals that while the two hydrophobins are similar, RodBp is not iinvolved in the rodlet formation but plays a role in the conidial cell wall structure. RodAp is required for rodlet formation as well as confers resistance to host cells against killing by alveolar macrophages.

United Kingdom, France, Analysis, Physiological aspects, Fungi, Plant cell walls, Cell walls, Fungal antigens

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Method for assessment of viability and morphological changes of bacteria in the early stage of colony formation on a simulated natural environment

Article Abstract:

A method was devised for measuring the viability and morphological changes of bacteria in the early stage of colony formation on a simulated natural environment. A solid medium containing soil extract was used in this method and the formation of bacterial microcolonies on a membrane filter was determined with the help of a laser scanning cytometer in combination with live-dead fluorescent dyes.

Japan, Usage, Microbiological techniques (Diagnosis)

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Glycosylphosphatidylinositol-anchored Ecm33p influences conidial cell wall biosynthesis in Aspergillus fumigatus

Article Abstract:

The disruption of the ECM33 gene in Aspergillus fumigatus (AfECM33) and the phenotype of the mutant are described. The analyses have demonstrated that mutation of the AfECM33 gene results in a conidium phenotype that is associated with a differential sensitivity of the mutant to phygocytes.

Methods, Genetic aspects, Aspergillus, Biosynthesis, Chemical properties, Phosphatidylinositol, Phosphatidylinositols

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