Conjugative transfer of chromosomal genes between fluorescent Pseudomonads in the rhizosphere of wheat

Article Abstract:

Pseudomonas aeruginosa and P. fluorescens strains containing plasmids with chromosome mobilizing ability, are capable of conjugatively transferring their chromosomal genes to auxotrophic recipient bacteria in wheat rhizosphere. The transfer occurs in natural nonsterile soil and under gnotobiotic conditions. No chromosomal gene transfer occurs in bulk soils. The study shows that natural and genetically introduced genes can be transferred between rhizosphere bacteria. However, the transfer in nature is limited by restriction and nonhomology of recipient bacteria's DNA.

Observations, Pseudomonas, Rhizosphere, Conjugation (Biology), Conjugation (Reproduction)

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Tn5-directed cloning of pqq genes from Pseudomonas fluorescens CHA0: mutational inactivation of the genes results in overproduction of the antibiotic pyoluteorin

Article Abstract:

Biosynthesis of the dehydrogenase cofactor pyrroquinoline quinone (PQQ) in Pseudomonas fluorescens CHA0 was done by cloning of the gene (pqq) that codes it. Cloning of a Tn5 insertion mutant that produces an excess of (pyoluteorine) Plt, but is unable to produce the antibiotic 2,4-diacetylphloroglucinol, indicates that the genes required for the synthesis of PQQ are inactivated by mutation. The gene product deduced (PqqFAB) is almost similar to the proteins involved in the biosynthesis of PQQ in Klebsiella, Acinobacter, and Methylobacterium.

Antibiotics, Genetic aspects, Gene expression, Microbial mutation

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Effect of starvation and the viable-but-nonculturable state on green fluorescent protein (GFP) fluorescence in GFP-tagged Pseudomonas fluorescens A506

Article Abstract:

Cells from Pseudomonas fluorescens that have been genetically altered with the gene for green fluorescent protein continue to fluoresce when starved and when in the viable-but-nonculturable state. However, dead cells gradually lose the protein, probably because of a breakdown in the cell membrane.

Fluorescence, Starvation

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