Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments

Article Abstract:

Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein substantially increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent characteristics for bioremediation of Hg2+-contaminated locations.

Methods, Escherichia coli, Bioremediation

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Cloning, expression, and characterization of cadmium and manganese uptake genes from Lactobacillus plantarum

Article Abstract:

Research conducted on the cloning of cadmium and manganese uptake gene from Lactobacillus plantarum is analyzed. Increase in cadmium uptake and the regulation of its expression are examined.

Cloning, Gene expression

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Characterization of cadmium uptake in Lactobacillus plantarum and isolation of cadmium and manganese uptake mutants

Article Abstract:

Research conducted on the chemical mutagenesis identification of two cadmium uptake systems is discussed. Experiments to isolate manganese dependent mutant strains are included.

Gene mutations, Gene mutation

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