Contraselectable streptomycin susceptibility determinant for genetic manipulation and analysis of Helicobacter pylori

Article Abstract:

A two-gene cassette that can be selected both, against, because of a Campylobacter jejuni rpsL gene and for, due to an erm gene (erythromycin resistance) is presented. The desired Erm (super s) STR (super r) were acquired at a range of frequencies from 17 to 96% among STr (super r) transformants, with the Erm (super s) yield apparently depending on the strain background and genome location of the targeted locus.

Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Missouri, Streptomycin Preparations, Streptomycin, Nucleotide sequencing

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Atypical helicobacter canadensis strains associated with swines

Article Abstract:

In The Netherlands and Denmark forty-two Helicobacter isolates were isolated from swine feces and all twelve isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis (99% similarity). Amplified fragment length polymorphism genomic typing showed that isolates recovered from swine feces were genetically distinct from H. canadensis strains obtained from humans and geese.

Denmark, Netherlands, Ribosomal RNA, Genetic polymorphisms

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Direct quantification of Campylobacter jejuni and Campylobacter lanienae in feces of cattle by real-time quantitative PCR

Article Abstract:

Campylobacter jejuni and Campylobacter lanienae in feces of cattle can be directly quantified using nested real-time quantitative (RTQ)-PCR method. The effectiveness of the RTQ-PCR method in quantifying Campylobacter jejuni and Campylobacter lanienae is proved using SYBR green.

Campylobacter

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