Crystal structure of the cys2 activator-binding domain of protein kinase C-delta in complex with phorbol ester

Article Abstract:

The binding domain for phorbol 13-acetate on the cys2 domain of protein kinase C-delta (PKC-delta) is formed by pulling apart the two beta-sheets and five hydrogen bonds are formed between the phorbol and PKC-delta. The binding of phorbol to the protein forms a hydrophobic surface which extends over 1/3 of the domain and the formation of the hydrogen bonds and the free energy released helps in the insertion of the protein into the membrane. The insertion of the unliganded protein into the membrane is unfavorable because hydrogen bonds at the membrane-protein interface are not formed.

Physiological aspects, Binding sites (Biochemistry), Active sites (Biochemistry), Phorbol esters

---

Structure of type II-beta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation

Article Abstract:

The structure of type II-beta phosphatidylinositol phosphate kinase (PIPK) shows a protein kinase ATP-binding core. It indicates that all phosphoinositide kinases are part of one superfamily. The flatness of the membrane-binding site, along with the shallowness of the substrate-binding pocket and its closeness to the membrane indicate that PIPKs phosphyorylate their substrates in situ in membranes. This allows the enzyme to avoid an energetic penalty for removing the substrate from its low-energy conformation within the membrane.

Research

---

Similar abstracts:

• Abstracts: Crystal structure of the replication terminator protein from B. subtilis at 2.6 angstrom. A 27 kd protein of E. coli promotes antitermination of replication in vitro at a sequence-specific replication terminus
• Abstracts: Differential effect of activators of protein kinase C on cytoskeletal changes in mouse and hamster eggs. Aberrant expression of Pax-2 in Danforth's short tail (Sd) mice
• Abstracts: Crystal structure of the Dbl and Pleckstrin homology domains from the Human Son of Sevenless protein. A myristoyl/phosphotyrosine switch regulates c-Abl
• Abstracts: Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid. Complement-independent, peroxide-induced antibody lysis of platelets in HIV-1-related immune thrombocytopenia
• Abstracts: ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. Microbial type I fatty acid synthases (FAS): major players in a network of cellular FAS systems

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.