Detection of Coxiella burnetii in cow's milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method

Article Abstract:

Sufficient concentration of Triton X-100 was observed to improve the immunomagnetic separation of Coxiella burnetii from milk. PCR-enzyme-linked immunosorbent assay (PCR-ELISA) can detect coxiellas more effectively than the standard PCR. PCR-ELISA is therefore believed to be applicable for the simultaneous assay of huge number of samples. However, the number of cows from which raw milk tested positive for coxiellas by PCR-ELISA failed to agree with that found with the antibody to coxiella by indirect immunofluorescence assay. This is due to the differences in the infectious route, infectious dose, or the timing of yielding the antibody and the period of duration of the antibody.

Dairy bacteriology

---

PCR detection of Coxiella burnetii from different clinical specimens, especially bovine milk, on the basis of DNA preparation with a silica matrix

Article Abstract:

A sample preparation method that uses silica binding of DNA for detecting polymerase reaction chain (PCR) of Coxiella burnetii from different clinical specimens is presented. The method was tested on more that 600 milk samples from 21 cows that carry the C. burnetii agent and have fertility problems. Results show that the method can detect C. burnetii in naturally infected specimens. However, the sensitivity of the method is inferior to other PCR-based detection methods.

Research, Usage, Polymerase chain reaction, Communicable diseases in animals, Communicable animal diseases

---

Similar abstracts:

• Abstracts: Detection of type A, B, E, and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies
• Abstracts: Forensic application of microbiological culture analysis to identify mail intentionally contaminated with bacillus anthracis spores
• Abstracts: Identification of the genetic basis for complex disorders by use of pooling-based genomewide single-nucleotide-polymorphism association studies
• Abstracts: The reduced expression of the HADH2 protein causes X-linked mental retardation, choreoathetosis, and abnormal behavior
• Abstracts: Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.