Detection of RTX toxin genes in gram-negative bacteria with a set of specific probes

Article Abstract:

A set of 10 gene probes to be employed for screening unidentified RTX toxin genes in bacterial species was determined by contrasting the genetic relationships of the RTX toxin genes. The probes detected all known genes for RTX toxins. Also, possible RTX toxin genes in a number of pathogenic bacterial species for which no toxins are yet known, have been found. This showed that RTX and RTX-like toxins are widely distributed among pathogenic gram-negative bacteria.

Genetic aspects, Gram-negative bacteria, Microbial toxins

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Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains

Article Abstract:

Escherichia coli (E, coli) B and C strains as well as the commercial strain TOPP are devoid of E. coli virulence genes and thus can be regarded as nonpathogenic. However, pathogenic E. coli control strains show typical hybridization patterns. The rational approach using probes and their application on a single filter can be used for the identification and characterization of clinically significant E. coli isolates from human and animal species.

Virulence (Microbiology)

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Rapid and accurate identification of Escherichia coli K-12 strains

Article Abstract:

A new polymerase chain reaction (PCR) method helps detect Escherichia (E.) coli K-12-derived strains. The strains are safe enough to be used in biotechnological research, development and production. The method involves the detection of K-12 strains by the structure of the O-antigen gene cluster of E. coli. The PCR routine can be applied for the second time based on the same gene cluster to obtain more clearly distinguished strains.

Usage, Polymerase chain reaction, Genetic screening, Genetic testing

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