Detection of cytolethal distending toxin activity and cdt genes in Campylobacter spp. isolated from chicken carcasses

Article Abstract:

The prevalence of cdt genes in fresh chicken carcasses was affirmed in a study of whether isolates from such carcasses commonly carry these genes and whether active cytolethal distending toxin is produced by these isolates. Biochemical and polymerase chain reaction tests confirmed the presence of Campylobacter spp. Seventy or 67% of the 105 isolates from 91 fresh chicken carcasses bought from local supermarkets were identified as C jejuni, while 35 or 33% were identified as C coli. These establish a high rate of Campylobacter spp contamination in fresh chicken carcasses and suggest that cdt genes may be universally present in C jejuni and C coli isolates from chicken carcasses.

Research, Meat, Chickens, Contamination, Food contamination, Microbial contamination

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Multivariate analysis of complex DNA sequences electropherograms for high-throughput quantitative analysis of mixed microbial populations

Article Abstract:

The use of multivariate statistical analysis of complex DNA sequence electropherograms for the effective and accurate estimation of relative genotype abundance in cell samples form mixed microbial population is described. Campylobacter jejuni strain specific marker gene gltA and 16S rRNA gene were used and in both cases the analysis provide time series population data demonstrating the usefulness of the method.

Analysis, Physiological aspects, Nucleotide sequence, Base sequence

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Concurrent quantitation of total Campylobacter and total ciprofloxacin-resistant Campylobacter loads in rinses from retail raw chicken carcasses from 2001 to 2003 by direct plating at 42 degree C.

Article Abstract:

A study about quantifying the persisting cipro-floxacin-resistant subpopulation of the total Campylobacter load on retail raw chicken carcasses was reported by using a direct plating method. It confirmed the presence and steady decline of subpopulations of ciprofloxacin-resistant Campylobacter jejuni and other Campylobacter spp. in retail raw chicken carcasses rinses.

Genetic research

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