Development of a sensitive chemiluminometric assay for the detection of beta-galactosidase in permeabilized coliform bacteria and comparison with fluorometry and colorimetry

Article Abstract:

A chemiluminometric assay detects the beta-galactosidase of coliform bacteria present in water quicker than fluorometry and colorimetry method. One coliform seeded in 100 ml of sterile water can be detected after a 6- to 9-hr propagation phase and a 45-min enzyme assay in the presence of polymixin B. The method can be used to test water samples during emergencies such natural disasters or failure of the water supply.

Usage, Measurement, Organic water pollutants, Organic water pollution, Microbial contamination, Chemiluminescence

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Limitations of highly sensitive enzymatic presence-absence tests for detection of waterborne coliforms and Escherichia coli

Article Abstract:

The enzymatic presence-absence tests fail to detect one waterborne coliform or one Escherichia coli in 100 ml of drinking water. Chemiluminometric procedures reveal that this limitation arises due to a compromise between the specificity of the test and the sensitivity-boosting measures that help to achieve the required speed. Isolation of the target bacteria can improve the specificity and sensitivity of the test.

Analysis, Evaluation, Drinking water, Microbiological assay

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Inhibition of Beta-galactosidase biosynthesis in Escherichia coli by tetracycline residues in milk

Article Abstract:

Milk tetracycline residues were found to inhibit Escherichia coli Beta-galactosidase biosynthesis. The same effect may be produced using other antibacterials, though these have to be in concentrations 10-fold higher. The findings were employed in developing a milk tetracycline screening test based on a Beta-galactosidase chemiluminometric assay. Contaminated samples of raw milk were used to verify the approach.

Research, Milk, Dairy bacteriology, Tetracycline, Tetracyclines

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