Differences in microbial activity and microbial populations of peat associated with suppression of damping-off disease caused by Pythium sylvaticum

Article Abstract:

A bioassay was used for Pythium sylvaticum-induced damping off of cress seedlings to identify conducive and suppressive peats. It was found at the end of the assay that microbial activity in unconditioned peats was negatively correlated with the counts of P. sylvaticum and there were also significant differences between bacterial sequence populations from suppressive and conducive peats.

Microbiological assay

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Identification of bacterial DNA markers for the detection of human fecal pollution in water

Article Abstract:

Genome fragment enrichment and bioinformatics are used for identifying several microbial DNA sequences with high potential for use as markers in PCR assays for the detection of human fecal contamination in water. The studies have shown the potential utility of these assays in detecting human fecal contamination in several impacted watersheds.

Genetic markers

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Self-formed adaptor PCR: a simple and efficient method for chromosome walking

Article Abstract:

A self-formed adaptor PCR (SEFA PCR) that can be used for chromosome walking was developed. Most of the amplified flanking sequences were found to be longer than 2.0kb and some were 6.0 kb which suggests that SEFA PCR is a simple and efficient method for the isolation of unknown sequences in complex genomes.

Chromosomes

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