Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure

Article Abstract:

Microwave radiation was used in Bacillus subtilis and Escherichia coli and found to bring a dramatic reduction in the viable counts, and E. coli cell surfaces were severely damaged, not the case for the B. subtilis cells. It has been popular for various uses in the food industry. The radiation also brought increases in amounts of DNA and protein released from the cells based on increase of final temperatures of the cell suspensions. No significant reduction of cell density was seen in the suspensions, probably because most of the bacterial cells inactivated did not lyse.

Escherichia coli, Bacterial cell walls, Microwaves

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Characterization of a metalloprotease inhibitor protein (SmaPI) of Serratia marcescens

Article Abstract:

Purification of the SmaPI metalloproteinase inhibitor from Serratia marcescens allowed biochemical characterization that revealed a high specificity for the S. marcescens SMP metalloproteinase. In S. marcescens containing a recombinant plasmid encoding the SmaPI and SMP, excess SmaPI was produced extracellularly and purified from the supernatant. The protein, which was monomeric and had a molecular mass of 10,000, was the product of cleavage of a precursor. It exhibited thermostability thought to result from reversible denaturation.

Analysis, Metalloenzymes, Inhibition, Inhibition (Psychology)

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Enhancement of secretion and extracellular stability of staphylokinase in Bacillus subtilis by wprA gene disruption

Article Abstract:

Results demonstrate that secretion and extracellular stability of staphylokinase in Bacillus subtilis can be enhanced by manipulating wprA gene. The gene codes protein codes a subtilisin-type protease which exhibits a significant role in the degradation of foreign proteins.

Statistical Data Included, Genetic aspects, Enzymes, Secretion, Physiological regulation, Plasminogen activators, Fibrinolytic agents, Enzyme synthesis

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