Differential susceptibility of aeromonads and coliforms to cefsulodin

Article Abstract:

Cefsulodin appears to be useful as a selective agent against Aeromonas spp. A combination of Chromocult Coliform (CC) agar and cefsulodin effectively suppresses the growth of Aeromonas and other oxidase-positive organisms from sewage-contaminated water samples. A concentration of 10 micro-g/ml or less of cefsulodin suppresses the growth of 96% of isolates. A comparison of the total coliforms (TCs) in CC agar with 10 mg of cefsulodin (CC-CFS) and without cefsulodin indicated no difference in Escherichia coli count in the two media. However, Aeromonas spp., which make up 58.6% of TCs on CC agar, were absent on CC-CFS.

Measurement, Environmental aspects, Microbial growth, Culture media (Biology), Culture media, Water pollution

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A selective and differential medium for Vibrio harveyi

Article Abstract:

Vibrio harveyi agar medium isolates and differentiates V. harveyi colonies from other Vibrio species. This medium inhibits the growth of two strains each of marine Pseudomonas and Flavobacterium but allows the growth of Photobacterium strains. The V. harveyi agar medium is more efficient than thiosulfate-citrate-bile salts-sugar mediums for primary isolation and identification of V. harveyi. The isolation of colonies displaying typical V. harveyi morphology with this agar in larval rearing water show positive results on analysis.

Methods, Analysis, Vibrio, Microbiological assay

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Bacterial growth state distinguished by single-cell protein profiling: does chlorination kill coliforms in municipal effluent?

Article Abstract:

Research was conducted to examine bacterial growth state in municipal effluent during wastewater disinfection. A novel immunofluorescence assay was developed and utilized for the study while eterobacterium-specific antibody fluorochrome-coupled probes were used to determine quantitative levels of three highly conserved cytosolic proteins. Results suggest that the current standard procedures for wastewater analysis, which depend on detection of culturable cells likely underestimate fecal coliform content.

Research, Bacterial growth, Sewage, Sewage microbiology, Chlorination, Single cell proteins

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