Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis

Article Abstract:

The display of a thermostable Bacillus sp. strain TG43 lipase in an active form on the Escherichia coli cell surface by employing FadL as an anchoring motif and its application in enantioselective biocatalysis are investigated. The examination of the enatioselective resolution of racemic methyl mandelate suggest that FadL may be a useful anchoring motif for displaying enzymes on the cell surface of E. coli for whole-cell biocatalysis.

Escherichia coli, Genetic research

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Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production

Article Abstract:

Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, Mannheimia succinicproducens, gene knockout studies were carried out to understand its anaerobic fermentative metabolism. An improved succinic acid producer could be developed by systematically disrupting the ldhA, pflB, pta, and ackA genes in the chromosome, which resulted in much reduced formation of common fermentation by-products.

Microbial metabolism, Bacteria, Chemical properties, Succinic acid

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Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applications

Article Abstract:

A new cell surface display system in Pseudomonas putida KT2442 is developed using OprF, an outer membrane protein of Pseudomonas aeruginosa, as an anchoring motif in a C-terminal deletion-fusion strategy. The results have demonstrated that the OprF anchor can be used for efficient display of proteins in Pseudomonas putida KT2442 and also for various biocatalytic applications.

Membrane proteins, Pseudomonas putida

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