Quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia

Article Abstract:

A quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-RFLP) assay, is developed for simultaneous determination of microbial diversity and abundance within a complex community. The results indicate that the real-time-t-RFLP assay can be a powerful molecular tool for learning about microbial communities in various engineered systems and natural habitats.

Genetic aspects, Bacillus thuringiensis, Genetic polymorphisms, Microbiological assay

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Molecular characterization of Brevibacillus lacterosporus and its potential use in biological control

Article Abstract:

Different PCR-based methods that were used to examine the genetic variability of thirty-three strains of Brevibacillus laterosporus including three novel strains isolated from Brazilian soil samples are discussed. Characterization of Brevibacillus laterosporus by different molecular methods reveals an elevated level of genetic polymorphism within the species, indicating that the isolates are clonally related.

Methods, Usage, Polymerase chain reaction, Bacterial genetics

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