Effect of L-proline on sake brewing and ethanol stress in Saccharomyces cerevisiae

Article Abstract:

The role of intracellular L-proline in cells of Saccharomyces cerevisiae grown under ethanol stress is evaluated. The strain that could accumulate L-proline was used to brew sake containing five times more L-proline than what is found in sake crewed with the control strain, without affecting the fermentation profiles.

United States, Industrial organic chemicals, not elsewhere classified, Ethanol, Ethyl Alcohol Manufacturing, Alcohol, Microbiological chemistry, Alcohol, Denatured

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Gene dosage effect of L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in Saccharomyces cerevisiae

Article Abstract:

The gene dosage effect of three L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in a non-L-proline-utilizing strain is examined. Overexpression of the wild-type enzymes has no influence on L-proline accumulation, which suggests that the complex is very unstable in nature.

Biosynthesis

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Desensitization of feedback inhibition of the Saccharomyces cervisiae [gamma]-glutamyl kinase enhances proline accumulation and freezing tolerance

Article Abstract:

Enzymatic analysis of recombinant proteins was carried out and Saccharomyces cervisiae was subjected to feedback inhibition by proline. It was seen that yeast cell expressing mutant [gamma]-glutamyl kinase were more tolerant to freezing stress than cells expressing the Asp154Asn mutant.

Genetic aspects, Structure, Phosphotransferases, Report

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