Effects of atrazine on Ochrobactrum anthropi membrane fatty acids

Article Abstract:

Cells of the gram-negative bacteria Ochrobactrum anthropi isolated from activated sludge show an increase in their membrane fatty acid saturation when grown on the pesticide atrazine. The bacteria use atrazine as the sole carbon source. Cells grown on atrazine contain 64% saturated fatty acids in their membranes while those grown on succinate contain 31%. The C16 and C18 fatty acids ratios increase while the concentrations of unsaturated fatty acids decrease in the presence of atrazine.

Analysis, Physiological aspects, Observations, Gram-negative bacteria, Fatty acids, Membranes (Biology), Atrazine

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Establishment of a gene expression system in Ochrobactrum anthropi

Article Abstract:

An enhanced gene expression system in Ochrobactrum anthropi was established to allow rapid and easy one-step purification of recombinant protein directly from Ochrobactrum anthropi. It was suggested that the newly described vectors will enhance genetic manipulation and characterization and will facilitate the use of Ochrobactrum as a potential bioremediation tool and a biopesticide agent.

Science & research, Research, Bioremediation, Bacterial genetics, Bacterial proteins, Structure

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L-Selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321

Article Abstract:

The purification, cloning and heterologous expression in Escherichia coli of the L-specific amidase from Ochrobactrum anthropi NCIMB 40321 wild-type cells are described. The results have shown that this amidase is responsible for the remarkably relaxed substrate specificity of this microorganism.

Genetic aspects, Escherichia coli, Serine, Chemical properties, Cysteine proteinases

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