Biotransformation of various substituted aromatic compounds to chiral dihydrodihydroxy derivatives

Article Abstract:

The chlorobenzene dioxygenase from Pseudomonas sp. strain P51 converts substituted aromatic compounds to the S configuration at the carbon atom. The enantiomeric excess was higher than the same reaction catalyzed by toluene dioxygenases. This is of interest to the biotechnology industry because cis-dihydrodiols are building blocks for molecules with multiple chiral centers.

Pseudomonas, Microbial enzymes

---

Enantioselective transformation of alpha-hexachlorocyclohexane by the dehydrochlorinases LinA1 and LinA2 from the soil bacterium Sphingomonas paucimobilis B90A

Article Abstract:

The enantioselective transformation of alpha-hexachlorocyclohexane (HCH) by LinA1 and LinA2 variants expressed as S-glutathione transferase (GST) fusion proteins in Escherichia coli BL21 is reported. Enantioselective degradation might be one of the key processes that determine enantiomer composition, especially when strains that contain only one of the linA genes, such as Sphingmonas paucimobilis UT26, prevail.

United States, Science & research, Pesticide and Other Agricultural Chemical Manufacturing, Agricultural chemicals, not elsewhere classified, Lindane, Escherichia coli, Soil microbiology, Bacterial proteins, Chemical properties

---

Enantioselective metabolism of chiral 3-phenylbutyric acid, an intermediate of linear alklybenzene degradation, by Rhodococcus rhodochrous PB1

Article Abstract:

The degradation of chiral 3-phenylbutyric acid by the gram-positive bacterium Rhodococcus rhodochorus PB1 was investigated. Only one enantiomer was used as a growth substrate. Data indicated that active cells were able to cometabolize the non-growth enantiomer to a dihydroxylated intermediate. Data also showed the importance of treating enantiomers separately in studies of chiral compound degradation.

Chirality, Decomposition (Chemistry)

Similar abstracts:

• Abstracts: Molecular characterization of CcpA and involvement of this protein in transcriptional regulation of lactate dehydrogenase and pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis
• Abstracts: Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa. Quantification of biofilm structures by the novel computer program COMSTAT
• Abstracts: Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii
• Abstracts: Effect of conventional and organic production practices on the prevalence and antimicrobial resistance of Campylobacter spp. in poultry
• Abstracts: Organization and regulation of meta cleavage pathway genes for toluene and o-xylene derivative degradation in Pseudomonas stutzeri OX1

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.