Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry
Article Abstract:
Flow cytometry measures bacterial biomass by correlating the total fluorescence intensity with the dry weight of a sample of cells grown in a pure culture simulating an aquifer microcosm. Flow cytometry provides a superior method for enumerating bacteria as compared to conventional acridine orange direct counting. Flow cytometry can be used for a very large sample size, and needs only one-tenth of the time required by acridine orange direct counting. Both the methods give statistically similar results.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
The freeze-thaw stress response of the yeast Saccharomyces cerevisiae is growth phase specific and is controlled by the nutritional state via the RAS-cyclic AMP signal transduction pathway
Article Abstract:
A study was conducted on the conditions that promote the survival of yeast cells subjected to freeze-thaw stress. The factors examined involve signal transduction pathways, prior stress treatments, growth phase and mitochondrial functions. Results show that relationship between viability loss and the duration of freezing, suggesting that freeze-thaw damage is due mainly to freezing.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
A flow cytometry method for rapid detection and enumeration of total bacteria in milk
Article Abstract:
Researchers describe a flow cytometry method that clears milk enzymatically of milk proteins and lipids. This provides an accurate count of the bacteria in the milk and the procedure takes less than one hour.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A theoretical and empirical investigation of the invasion dynamics of colicinogeny. Can phage defence maintain colicin plasmids in Escherichia coli?
- Abstracts: Purification and characterization of an intracellular peroxidase from Streptomyces cyaneus. Purification and characterization of 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2
- Abstracts: Isolation and regulation of Sinorhizobium meliloti 1021 loci induced by oxygen limitation. Transcriptional organization and regulation of a polycistronic cold shock operon in Sinorhizobium meliloti RM1021 encoding homologs of the Escherichia coli major cold shock gene cspA and ribosomal protein gene rpsU
- Abstracts: Extended survival and persistence of Campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rRNA staining
- Abstracts: Evidence for a role for the gumB and gumC gene products in the formation of xanthan from its pentasaccharide repeating unit by Xanthomonas campestris