Enumeration of transconjugated Ruminococcus albus and its survival in the goat rumen microcosm

Article Abstract:

The mutant strain Ruminococcus albus A3 was introduced into the rumen of a goat and its survival was tested to determine how long antibiotic-resistant cellulose using bacteria can survive in the rumen. The number of bacteria decreased sharply after one day possibly because of the presence of protozoan predators and a low growth rate. In the rumen of the goat, the bacteria survived for 14 days, while in a simulated rumen, the bacteria survived for only 96 hours and the number of surviving cells was less.

Health aspects, Ruminants, Rumen, Rumen microbiology

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Phenylacetic and phenylpropionic acids do not affect xylan degradation by Ruminococcus albus

Article Abstract:

The addition of either ruminal fluid or a combination of phenylacetic and phenylpropionic acids (PAA/PPA) is shown to dramatically improve cellulose degradation and growth of Ruminococcus albus. The results show that the xylanolytic potential of Ruminococcus albus is not conditional on the availability of PAA/PPA or other components of ruminal fluid.

Science & research, Research, Cellulose, Plant cell walls

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Genomic differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, identified by suppression subtractive hybridization

Article Abstract:

Suppression hybridization is used for identifying the genes present in Fibrobacter succinogenes S85 but absent from Fibrobacter intestinalis DR7. Two gene clusters encoding NADH-ubiquinone oxidoreductase subunits shared by Fibrobacter genera have played a vital role in energy metabolism.

Analysis, Genetic aspects, Energy metabolism, Oxidoreductases, Bioenergetics, Ubiquinones, Chemical properties

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