Use of propidium monoazide for live/dead distinction in microbial ecology

Article Abstract:

Four experiments performed to study the usefulness of propidium monoazide (PMA) treatment of mixed bacterial communities comprising intact and compromised cells in combination with end-point PCR. The two experiments suggest that PMA treatment affects banding patterns in denaturing gradient gel electrophoresis community profiles.

Analysis, Gel electrophoresis, Microbial ecology

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Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples

Article Abstract:

A new concept is developed with the viable/dead stain ethidium monoazide (EMA) in combination with real-timePCR (EMA-PCR). EMA-PCR offers a novel real-time PCR method for quantitative distinction between viable and dead cells with potentially very wide application.

Science & research, Research, Cells (Biology), Cells

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