Expression cloning of 2-5A-dependent RNAase: a uniquely regulated mediator of interferon action

Article Abstract:

The expression cloning and analysis of murine and human 2-5A-dependent RNAases is reported. 2-5A-dependent RNAase is an interferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A). It is implicated in the molecular mechanisms of interferon action and the control of RNA stability in mammalian cells. The results showed similarity of 2-5A-dependent RNAase to RNAase E, an enzyme involved in mRNA stability in Escherichia coli. Deletion analysis and site-directed mutagenesis showed that a duplicated phosphate-binding loop motif is involved in 2-5A binding.

Research, Enzymes, Interferon

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Identification of ligands for olfactory receptors by functional expression of a receptor library

Article Abstract:

The interaction between odorants and olfactory receptors was examined using an olfactory receptor library expression. A total of 80 chimeric receptors from this library were evaluated against 26 odorants after being transfected into HEK-293 cells. The analysis identified three receptors that responded to micromolecular concentrations of carvone, (-)citronellal and limonene. The study also revealed that the mouse 17 receptor favors heptanal instead ofoctanal due to a single valine-to-isoleucine substitution.

Ligands (Biochemistry), Odors, Neural receptors

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Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation

Article Abstract:

A novel member of the otx homeodomain gene family, Crx, was isolated and characterized through reporter constructs for a transactivation assay of photoreceptor cells in the neural retina. Results revealed that CRX exhibited specific binding to conserved DNA sequences found upstream of several photoreceptor-specific genes. CRX was able to activate transcription from the minimum promoter in a CRX-binding site-dependent manner.

Development and progression, Retinal degeneration, Photoreceptors

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