Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae

Article Abstract:

The Trichoderma reesei beta-xylanase (XYN2) gene in Saccharomyces cerevisiae has been molecularly cloned. Multicopy plasmids using two Saccharomyces cerevisiae promoter-terminator expression cassettes aided in the expression of the XYN2 gene. Results supported the use of beta-cylanase for industrial enzyme production, even if its production by recombinant organisms m,ay not be as high as that in organisms such as the Trichoderma reesei strain.

Genetic aspects, Fungi, Gene expression, Saccharomyces

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Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes

Article Abstract:

Researchers successfully bioengineered Saccharomyces cerevisiae with the beta-xylosidase gene of Aspergillus niger 90196 and the beta-xylanase gene from Trichoderma reesei. The coproduction of these two fungal enzymes allowing the yeast to degrade xylan. Beta-xylanases have industrial applications in the food and biobleaching industries.

Microbial enzymes

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Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes

Article Abstract:

Saccharomyces cerevisiae can create ethanol from xylose if it contains the xylA gene from Thermus thermophilus and the GRE3 yeast gene is removed. Xylose is a common sugar in lignocellulosic material, which is fermented to produce ethanol as an alternative to fossil fuels.

Biomass energy

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