Expression of the eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp

Article Abstract:

Metabolic engineering research has developed a gene clone of the eicosapentaenoic acid synthesis gene cluster from the bacterium Shewanella putrefaciens strain SCRC-2738 and expressed the gene in the marine cyanobacterium Synechococcus species. The cloning technique involved the use of broad-host-range cosmid vector pJRD215 to create plasmid pJRDEPA. Cyanobacterial transconjugants were grown at 23 degrees centigrade and incubated at 17 degrees centigrade for 24 hours.

Methods, Cyanobacteria, Marine bacteria, Cloning, Microbial biotechnology

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Assembly of G protein-coupled receptors onto nanosized bacterial magnetic particles using Mms16 as an anchor molecule

Article Abstract:

GPCRs have been successfully assembled into the lipid membrane of nanosized bacterial magnetic particles (BMPs) produced by the magnetic bacterium magnetospirllum magneticum AMB-1. The system conveniently refines the native conformation of GPCRs without the need for detergent solubilization, purification, and reconstruction after cell desruption.

Science & research, Research, Risk factors, Microbiology, Proteins, Lipid membranes

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Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum

Article Abstract:

This article describes the development of a shuttle vector, pUMG, from a 3.7-kilobase cryptic plasmid, pMGT, discovered in Magnetospirillum magneticum. The shuttle vector is an improved expression system than that of pMGT constructed in M. magneticum strain AMB-1.

Japan, Design and construction, Plasmids, Magnetic properties

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