Monitoring genome evolution ex vivo: reversible chromosomal integration of a 106 kb plasmid at two tRNA(super.Lys) gene loci in sequential Pseudomonas aeruginosa airway isolates

Article Abstract:

Monitoring genome evolution in Pseudomonas aeruginosa ex vivo is discussed. Reversible chromosomal integration of a 106-kb plasmid at two tRNA(super.Lys) gene loci in sequential airway isolates from a cystic fibrosis patient was carried out. The example shows the potential systematic genome analysis of sequential isolates from the same habitat on understanding of the evolution of microbial genomes.

Germany, Causes of, Plasmids, Genetic recombination, Transfer RNA, Variation (Biology), Cystic fibrosis

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Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes

Article Abstract:

Phylogeny of the genus Pseudomonas is discussed with attention to intrageneric structure derived based on the nucleotide sequences of gyrB and rpoD genes and phylogenetic analysis. Classification criteria for Pseudomonas as a genus have been revised. Two major clusters, intrageneric clusterI and intrageneric cluster II, have emerged from the study.

Pseudomonas, Polymerase chain reaction, Phylogeny, Cladistic analysis

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Expression of the ftsY gene, encoding a homologue of the alpha subunit of mammalian signal recognition particle receptor, is controlled by different promoters in vegetative and sporulating cells of Bacillus subtilis.

Article Abstract:

The Bacillus subtilis gene ftsY is expressed only at t(sub.8) during sporulation and is from a promoter controlled by sigma(super.K) and GerE and immediately upstream of ftsY and inside the smc gene. B. subtilis FtsY (Srb) is a homolog of the alpha subunit of the receptor for the mammalian signal-recognition particle and is required for vegetative cell growth and protein secretion. To look at the role of FtsY in sporulation, the B. subtilis strain ISR39 ws established. It is an ftsY conditional mutant in which ftsY expression can be shut off during spore formation but not during the vegetative stage.

Physiological aspects, Spores (Bacteria), Bacillus subtilis, Bacterial spores, Genetic regulation, Gene expression, Cellular control mechanisms, Cell regulation, Electron microscopy, Cell receptors, Mammals

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